Extended Data Fig. 8. Reduction of CDK9 kinase activity and CDK9 expression within the nucleus is not due to increased degradation.
(a) Real-time PCR analysis of endogenous CDK9 mRNA expression in SKOV3 cells treated with or without ALK inhibitor. Data are represented as mean ± SD of N = 3 independent experiments. n.s., not significant, two-tailed unpaired t test. (b, c) p-T186 expression (b) and subcellular localization (c) of Flag-tagged CDK9 in cells expressing Y19F and WT CDK9. (d) Subcellular localization of CDK9 in cells expressing WT CDK9 treated with or without ALKi. Data are representative of two repeats with similar results. Cells were treated with 10 μΜ proteasome inhibitor (MG132) for 3 h prior to WB analysis (b–d).