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. 2022 Oct 21;13(10):890. doi: 10.1038/s41419-022-05321-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — 3T3-L1 preadipocytes or iWAT-SVF were treated with LLY-507 and meanwhile differentiated into mature adipocytes. A The Oil Red O staining of 3T3-L1 adipocytes. Magnification ×200. B The mRNA levels of adipogenesis markers (Pparγ, Cepbα, and Fabp4) in 3T3-L1 adipocytes. C The protein expressions of adipogenesis markers (PPARγ, C/EBPα, and FABP4) in 3T3-L1 adipocytes treated with LLY-507 in a dose-dependent manner. D The Oil Red O staining of iWAT-SVF differentiated adipocytes. Magnification ×400. E The mRNA levels of adipogenesis markers (Pparγ, Cepbα and Fabp4) in iWAT-SVF differentiated adipocytes. F The protein expressions of adipogenesis markers (PPARγ, C/EBPα and FABP4) and the rate-limiting enzyme in adipogenesis (ACC and FASN) in iWAT-SVF differentiated adipocytes. Data are presented as mean ± SD. **p < 0.01, ***p < 0.001.