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. 2022 Oct 21;13(10):890. doi: 10.1038/s41419-022-05321-7

Fig. 5. Smyd2 regulates adipocyte differentiation via phosphorylating STAT3.

Fig. 5

A, B The phosphorylation levels of STAT3 (Tyr705), as well as the unphosphorylated form, were detected by western blot in either Smyd2 siRNA- or LLY-507-treated 3T3-L1 and iWAT-SVF adipocytes. C The RT-qPCR analysis of the adipogenesis marker genes (Pparγ, Cepbα, and Fabp4) in Stattic (5 μM)-treated 3T3-L1 adipocytes. D The PPARγ protein expression was detected by western blot upon Smyd2 siRNA transfection or Smyd2 siRNA in combination with Stattic (5 μM) treatment. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.