Table 1.
Strains and plasmids used in this study
| Strain or Plasmid | Genotype or Relevant Characteristics | Source or Reference |
|---|---|---|
| Strain | ||
| Saccharomycescerevisiae | ||
| RS453 | MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-52 | (81) |
| SS1026 | pah1Δ::TRP1 derivative of RS453 | (61) |
| Escherichia coli | ||
| BL21(DE3)pLysS | F-ompT hsdSB (rB-mB-) gal dcm (DE3) pLysS | Novagen |
| BL21(DE3) | F-ompT hsdSB (rB-mB-) gal dcm (DE3) | Invitrogen |
| Plasmid | ||
| YCplac111-GAL1/10-NEM1-PtA | NEM1-PtA under control of GAL1/10 promoter inserted in CEN/LEU2 plasmid | (61) |
| pRS313-GAL1/10-SPO7 | SPO7 under control of GAL1/10 promoter inserted in CEN/HIS3 plasmid | (82) |
| pGH313 | PAH1 coding sequence inserted into pET-15b | (35) |
| pGH313-D398E | pGH313 containing the D398E mutation in the PAH1 coding sequence | (83) |
| EB1164 | PHO85-His6 derivative of pQE-60 | (84) |
| EB1076 | PHO80 derivative of Psbeta | (84) |