Skip to main content
. 2022 Sep 15;298(11):102497. doi: 10.1016/j.jbc.2022.102497

Figure 8.

Figure 8

UA inhibits the mobility of the FcεRI and cholesterol, as determined by FRAP.A and B, mobility of the FcεRI-IgE-FITC complexes. A, RBL-2H3 cells were exposed for 15 min to 50 μM UA in DMSO (UA) or the corresponding concentration of vehicle (Ctrl). Then the cells were exposed to IgE-FITC complexes. Image sequences are from cells at the indicated time points before photobleaching (Pre-bleach) at photobleaching (Bleach) or after photobleaching (Post-bleach; the circled areas). The graph in (B) quantifies the recovery into the photobleached areas. Means ± SEMs were calculated from 18 cells in two independent experiments with similar results. C and D, mobility of the cholesterol–Filipin complexes. C, RBL-2H3 cells were exposed for 15 min to 50 μM UA in DMSO or the corresponding concentration of vehicle (Ctrl). Then the cells were exposed to filipin at 50 μg/ml for 10 min. After washing, the image sequences from cells at the indicated time points before, at, or after photobleaching of the circled areas. The graph in (D) quantifies the recovery into the photobleached region. Means ± SEMs were calculated from 15 cells in two independent experiments with similar results. Statistical significance of differences between control (Ctrl) and UA-treated cells (UA) is also indicated. Bar = 10 μm. DMSO, dimethyl sulfoxide; UA, ursolic acid.