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. 2022 Oct 21;12:17658. doi: 10.1038/s41598-022-21678-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of N-glycosylation competent or deficient M₃ and SST₃ signal sequences on the functional responses of correspondingly tagged TAS2R20/38/50 upon stimulation by their cognate agonists. TAS2R20/38/50 grafted with N-glycosylation-deficient M₃ and SST₃ signal sequences were resistant to activation by their cognate agonists. Data points are shown as mean ± s.e.m. from a representative experiment out of three independent biological replicates performed in technical quadruplicates.