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. 2022 Sep 20;298(11):102513. doi: 10.1016/j.jbc.2022.102513

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

14-3-3 are relocalized in HCMV-infected cells independently of UL97 association.A, serum-starved hTERT-BJ1 fibroblasts were mock infected or infected with the indicated recombinant viruses at an MOI of 3. At 4 days post-infection (dpi), cells were subjected to immunostaining with anti-HA and anti-14-3-3ε antibodies. DNA is stained with DAPI. B, indirect immunofluorescence as in panel (A) with a 14-3-3γ antibody. C, indirect immunofluorescence as in panel (A) with a 14-3-3θ antibody. Blue: image for DAPI. Red: image for anti-HA antibody. Green: image for anti-14-3-3 antibody. Experiments were performed in duplicate. DAPI, 4′,6-diamidino-2-phenylindole; MOI, multiplicity of infection.