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. 2022 Sep 20;298(11):102513. doi: 10.1016/j.jbc.2022.102513

Figure 13.

Figure 13

14-3-3 binding by UL97 minimally effects maribavir sensitivity but not ganciclovir sensitivity.A, serum-starved hTERT-BJ1 fibroblasts were infected with the indicated recombinant AD169-based viruses at an MOI of 0.1. Ganciclovir (GCV, 2 μM) or DMSO were added after 1 h. Cell-free virus was collected at 9 days post-infection (dpi) and titers were determined by plaque assay. The value shows virus titer relative to rBAD-HA97 with GCV. B, analysis as in panel (A) except in the presence or absence of maribavir (MBV, 2 μM). The value shows virus titer relative to rBAD-HA97 with MBV. C, serum-starved hTERT-BJ1 fibroblasts were infected with the indicated recombinant TB40/E-based viruses at an MOI of 0.05. GCV (2 μM) or DMSO were added after 1 h. Cell-free virus was collected at 9 days post-infection (dpi) and titers were determined by plaque assay as in panel (A). The value shows virus titer relative to rTB40/E-HA97 with GCV. D, analysis as in panel (C) except in the presence or absence of MBV (2 μM). The value shows virus titer relative to rTB40/E-HA97 with MBV. Error bars denote SDs from three biological replicates. Statistical analysis utilized a two-tailed unpaired Student’s t test. n.s., not significant (p > 0.05). DMSO, dimethyl sulfoxide; MOI, multiplicity of infection.