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. 2022 Sep 20;298(11):102513. doi: 10.1016/j.jbc.2022.102513

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Serine 13 is not required for the kinase activity of transfected UL97. Saos-2 cells were transfected for 48 h with the indicated amounts (μg) of an expression vector for WT UL97 or the S13A mutant together with expression vectors for Flag-tagged RB and the indicated Flag-tagged 14-3-3 isoform. Differing amounts of expression vector for WT UL97 or the S13A mutant of UL97 were used. Lysates were analyzed by Western blotting with the indicated antibodies. A representative image from one of three independent biological replicates is shown.