UL97 Serine-13 is required for interaction with 14-3-3 proteins during HCMV infection.A, serum-starved hTERT-BJ1 fibroblasts were infected with the indicated recombinant HCMVs at an MOI of 3. At 4 days post-infection (dpi), lysates (Input) were harvested, subjected to immunoprecipitation with an anti-HA antibody (IP: anti-HA), and analyzed by Western blot with the indicated antibodies. B, serum-starved hTERT-BJ1 fibroblasts were infected as in panel (A). At 4 dpi, lysates (Input) were harvested, subjected to immunoprecipitation with the indicated 14-3-3 antibody (IP: anti-14-3-3) or rabbit IgG, and analyzed by Western blot with the indicated antibodies. For all panels, representative images from one of three independent biological replicates are shown. MOI, multiplicity of infection.