Table 2.
Autophosphorylation sites and predicted 14-3-3 binding sites on UL97
| Ser/Thr | Surrounding sequencea | Autophosphorylationb | ANNc | PSSMd | SVMe |
|---|---|---|---|---|---|
| Ser-2 | MS∗SALRS | + | − | − | − |
| Ser-3 | MSS∗ALRSR | + | − | − | − |
| Ser-11 | RSRARS∗ASLGT | + | + | + | − |
| Ser-13 | RARSAS∗LGTTT | + | + | + | + |
| Thr-16 | SASLGT∗TTQGW | + | − | − | − |
| Thr-18 | SLGTTT∗QGWDP | + | − | − | − |
| Ser-133 | RPVVPS∗TSSRG | + | − | − | − |
| Thr-134 | PVVPST∗SSRGS | + | − | − | − |
| Thr-154 | LRCRET∗SAMWS | − | + | − | − |
| Ser-155 | RCRETS∗AMWSF | − | + | + | − |
| Thr-177 | RRALFT∗GGSDP | + | − | − | − |
| Thr-298 | MFLRLT∗HPELC | − | + | + | − |
| Ser-361 | VARKHS∗ETVLT | − | + | + | + |
| Thr-409 | LLHNVT∗VHRRF | − | + | − | − |
| Thr-416 | HRRFHT∗DMFHH | − | + | + | − |
| Thr-689 | AFRRTT∗SIICE | − | + | + | − |
| Ser-690 | FRRTTS∗IICEE | − | + | + | + |
a
Ser/Thr sites (∗) and surrounding sequence.
b
+, known autophosphorylation site.
c
ANN, Artificial Neural Network (cut-off = 0.55).
d
PSSM, Position-Specific Scoring Matrix (cut-off = 0.80).
e
SVM, Support Vector Machine (cut-off =0.55).