Table 2.
GeNorm result represented with M-values and corresponding stability ranking for different analytic approaches compared with the pre-selection.
Reference gene candidates | Pre-selection | Physiologic condition (FiO2 = 0.21, PND2.5–28.5, RIN > 5; n = 17) | Developmental stages (FiO2 = 0.21, RIN > 5) | Sex (FiO2 = 0.21, PND2.5–28.5, RIN > 5) | Low RNA quality (FiO2 = 0.21, PND2.5–28.5, RIN < 5; n = 33) | Hyperoxia exposure (FiO2 = 0.8, PND2.5–28.5, RIN > 5; n = 36) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PND2.5 (n = 6) | PND5.5 (n = 7) | PND14.5–28.5 (n = 4) | Male (n = 31) | Female (n = 29) | ||||||||||||||
Overall SD | Rank | M-value | Rank | M-value | Rank | M-value | Rank | M-value | Rank | M-value | Rank | M-value | Rank | M-value | Rank | M-value | Rank | |
Auh | 0.151 | 8 | 0.541 | 10 | 0.204 | 8 | 0.463 | 13 | 0.480 | 16 | 0.392 | 8 | 0.397 | 7 | 1.537 | 22 | 0.299 | 1 |
Csnk1a1 | 0.199 | 19 | 0.375 | 3 | 0.317 | 17 | 0.382 | 9 | 0.420 | 11 | 0.231 | 1 | 0.224 | 1 | 0.908 | 11 | 0.407 | 5 |
Dolpp1 | 0.113 | 1 | 0.498 | 7 | 0.125 | 4 | 0.132 | 1 | 0.558 | 21 | 0.416 | 10 | 0.418 | 8 | 0.640 | 5 | 0.372 | 4 |
Efhd2 | 0.157 | 9 | 0.589 | 13 | 0.265 | 13 | 0.295 | 5 | 0.532 | 20 | 0.433 | 12 | 0.465 | 11 | 0.585 | 4 | 0.601 | 13 |
Eif3a | 0.197 | 16 | 0.431 | 5 | 0.355 | 20 | 0.429 | 12 | 0.490 | 17 | 0.333 | 5 | 0.510 | 15 | 0.541 | 3 | 0.535 | 10 |
Eif4g2 | 0.198 | 17 | 0.836 | 20 | 0.236 | 11 | 0.619 | 17 | 0.347 | 8 | 0.231 | 1 | 0.312 | 4 | 0.469 | 1 | 0.583 | 12 |
Elac2 | 0.119 | 4 | 0.468 | 6 | 0.061 | 1 | 0.284 | 4 | 0.303 | 7 | 0.425 | 11 | 0.438 | 9 | 1.099 | 15 | 0.496 | 8 |
Grn | 0.191 | 14 | 0.937 | 22 | 0.097 | 3 | 0.814 | 21 | 0.376 | 9 | 0.446 | 14 | 0.478 | 12 | 1.259 | 18 | 0.890 | 22 |
Kdm3b | 0.121 | 5 | 0.555 | 11 | 0.165 | 6 | 0.253 | 3 | 0.500 | 18 | 0.476 | 17 | 0.520 | 16 | 0.469 | 1 | 0.745 | 18 |
Maea | 0.137 | 6 | 0.401 | 4 | 0.292 | 15 | 0.412 | 11 | 0.239 | 5 | 0.369 | 7 | 0.490 | 13 | 0.669 | 6 | 0.436 | 6 |
Mpv17I | 0.113 | 2 | 1.053 | 23 | 0.509 | 23 | 0.966 | 23 | 0.737 | 23 | 0.719 | 23 | 0.782 | 23 | 1.619 | 23 | 0.950 | 23 |
Nupl2 | 0.178 | 12 | 0.280 | 1 | 0.141 | 5 | 0.314 | 6 | 0.221 | 4 | 0.451 | 15 | 0.453 | 10 | 0.766 | 8 | 0.510 | 9 |
Pcsk7 | 0.118 | 3 | 0.513 | 8 | 0.061 | 1 | 0.132 | 1 | 0.514 | 19 | 0.406 | 9 | 0.542 | 18 | 0.868 | 10 | 0.474 | 7 |
Psmd4 | 0.358 | 22 | 0.568 | 12 | 0.278 | 14 | 0.334 | 7 | 0.189 | 3 | 0.440 | 13 | 0.575 | 20 | 0.704 | 7 | 0.562 | 11 |
Puf60 | 0.312 | 20 | 0.884 | 21 | 0.414 | 22 | 0.881 | 22 | 0.586 | 22 | 0.615 | 22 | 0.667 | 22 | 1.453 | 21 | 0.843 | 21 |
Rexo5 | 0.166 | 11 | 0.714 | 16 | 0.343 | 19 | 0.505 | 14 | 0.401 | 10 | 0.514 | 19 | 0.597 | 21 | 0.971 | 12 | 0.773 | 19 |
Rpl4 | 0.491 | 23 | 0.280 | 1 | 0.222 | 10 | 0.360 | 8 | 0.152 | 1 | 0.535 | 20 | 0.531 | 17 | 1.320 | 19 | 0.340 | 3 |
Tbp | 0.194 | 15 | 0.637 | 14 | 0.215 | 9 | 0.551 | 15 | 0.469 | 15 | 0.265 | 3 | 0.269 | 3 | 1.138 | 16 | 0.669 | 15 |
Tfrc | 0.333 | 21 | 0.778 | 18 | 0.304 | 16 | 0.771 | 20 | 0.261 | 6 | 0.354 | 6 | 0.337 | 5 | 1.016 | 13 | 0.722 | 17 |
Tmed2 | 0.185 | 13 | 0.806 | 19 | 0.248 | 12 | 0.723 | 19 | 0.152 | 1 | 0.566 | 21 | 0.224 | 1 | 1.196 | 17 | 0.797 | 20 |
Tnks2 | 0.198 | 18 | 0.748 | 17 | 0.372 | 21 | 0.665 | 18 | 0.444 | 13 | 0.462 | 16 | 0.555 | 19 | 1.059 | 14 | 0.700 | 16 |
Wars2 | 0.150 | 7 | 0.676 | 15 | 0.189 | 7 | 0.585 | 16 | 0.459 | 14 | 0.492 | 18 | 0.500 | 14 | 1.384 | 20 | 0.631 | 14 |
Zc3h11a | 0.166 | 10 | 0.528 | 9 | 0.331 | 18 | 0.400 | 10 | 0.432 | 12 | 0.313 | 4 | 0.368 | 6 | 0.827 | 9 | 0.299 | 1 |