Fig. 5.

Impact of HAS2-depletion on the sphere formation capacity of SKOV3 cells. Hanging drop method was used to show differences in cell cohesion and sphere formation capability of HAS2 knockdown SKOV3 cells compared to control SKOV3 cells. a, b Area and perimeter of the spheres excluding the diffuse edge. The area or perimeter of spheres of HAS2 knockdown and control cells was measured at day 4 and day 7. *p ≤ 0.05, c Representative pictures of spheres in drops of HAS2 knockdown cells and control cells. Note presence of a solid dark core and a light, diffuse edge. The values were built out of data of 4 experiments á 12 drops for HAS2 knockdown and control cells (n = 48). AU arbitrary unit