Fig. 1.

M₁ expression is decreased in temporal cortex samples from 40 Rett syndrome (RTT) autopsy samples. A–B Western blot. mAChR (M₁–M₅) protein levels are significantly decreased in the brain samples (BA38) from RTT autopsies when compared relative to age, sex, and postmortem interval matched controls (N = 12). Student’s t test. C–D qRT-PCR. mRNA expression of the mAChR subtype M₁ receptor is significantly decreased in human RTT samples, while M₄ levels are comparable to controls in the temporal cortex. Student’s t test. E–F Linear regression analysis. A correlative analysis shows a significant linear relationship between M₁ and MeCP2 transcript levels, which is not observed with M₄. Linear regression. G Western blot. Relative to Mecp2^+/+ controls, synaptosome preparations from 20-week-old Mecp2^+/- mice show a significant reduction in mAChR levels in the brainstem. Two-way ANOVA with Tukey post hoc analysis. N = 5/treatment/genotype. *p < 0.05, **p < 0.01