Fig. 2. Knockdown of BUD31 induces spontaneous apoptosis in ovarian cancer cells.

GO biological process enrichment (a) and Circos plot (b) analysis were conducted on DEGs in the RNA-seq data from HEYA8 cells after BUD31 knockdown. c GSEA analysis was performed with the gene expression profile after BUD31 knockdown (Normalized ES = –1.222, p < 0.001). d, e Apoptotic cells were detected by flow cytometry after staining with Annexin V-PE/7-AAD in ovarian cancer cells after BUD31 knockdown with two strands of siRNAs (HEYA8 and OV90) or overexpression (HEYA8 and A2780) (n = 3 biologically independent experiments). Apoptotic cells percentage included early and late apoptotic cells. Cells overexpressing BUD31 were treated with H₂O₂ with a final concentration of 400 μM for 4 h before apoptosis detection. f Apoptotic markers were measured by western blot. BUD31 was knocked down in HEYA8 and OV90 cells and was overexpressed HEYA8 and A2780 cells in the presence of H₂O₂. The p value was obtained by two-tailed unpaired Student’s t-test (e, f), and data are presented as means ± SD. Source data are provided as a Source Data file.