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. 2022 Oct 21;5:1118. doi: 10.1038/s42003-022-04087-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Fluorescence of exponential phase BY4741 S. cerevisiae cells harbouring the plasmids pGREG576_TEF_CgHXT4/6/7 (CgHxt4/6/7_GFP) or pGREG576_TEF_CgHXT6/7 (CgHxt6/7_GFP), and KUE100::URA- C. glabrata cells harbouring the plasmids pGREG576_PDC1_CgHXT4/6/7 (CgHxt4/6/7_GFP) or pGREG576_PDC1_CgHXT6/7 (CgHxt6/7_GFP), after 6 h of constitutive recombinant protein production. b Comparison of spot growth assays of the EBY.VW4000 (Schxt⁰) strain harbouring the pGREG567_TEF vector or the pGREG576_TEF_CgHXT4/6/7 or pGREG576_TEF_CgHXT6/7 expression plasmids in the presence of different indicated hexoses as carbon sources. The inocula were prepared as described in the Methods section. Cell suspensions used to prepare the spots were 1:5 (b) and 1:25 (c) dilutions of the cell suspensions used in (a). The displayed images are representative of at least three independent experiments.