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. 2022 Oct 21;5:1118. doi: 10.1038/s42003-022-04087-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Comparison of the susceptibility to antifungal azole drugs, at the indicated concentrations, of the C. glabrata KEU100 wild-type strain or derived KUE100_Δcghxt4/6/7, KUE100_Δcghxt6/7 or KUE100_Δcghxt4/6/7Δcghxt6/7 mutant cells on YPD agar plates by spot assays. The inoculum was prepared as described in the Methods section. Cell suspensions used to prepare the spots correspond to 1:5 (b) and 1:25 (c) dilutions of the cell suspensions used in (a). The displayed images are representative of at least three independent experiments. b Time-course accumulation ratio of [³H]-Fluconazole in non-adapted KUE100 wild-type cells (black circles) or derived KUE100_Δcghxt4/6/7 mutant cells (grey squares) during cultivation in liquid YPD medium in the presence of unlabelled fluconazole. The accumulation ratio values are averages of, at least, n = 3 independent experiments. Error bars represent the corresponding standard deviations. Significance levels are attributed as follows: *p value < 0.05; **p value < 0.01; ***p value < 0.001.