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. 2022 Oct 17;85:104304. doi: 10.1016/j.ebiom.2022.104304

Figure 3.

Figure 3

MRG-229 reverses fibrosis in a human Precision-Cut Lung Slice (hPCLS) model (blinded experiment). (a) RT-PCR analysis of COL1A1 relative expression (b) RT-PCR analysis of COL3A1 relative expression. (c) RT-PCR analysis of miR-29 level in human PCLS after 120 hrs in media + FC. (d) Masson Trichrome on human PCLS from healthy donors, cultured with either a fibrosis-inducing cocktail or vehicle for 120 hrs with daily media changes, treated with a peptide or a cholesterol-conjugated MRG-229 mimic. (e) Graph depicts quantification of collagen levels after 120 hrs on histology. Statistical analyses Ordinary one-way ANOVA (***P<0.001) were performed in GraphPad Prism.