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. 2022 Oct 17;85:104304. doi: 10.1016/j.ebiom.2022.104304

Figure 4.

Figure 4

Day 14 analysis of prophylactic dosing of MRG-201 at 100 mpk and peptide-conjugated MRG-229 mimic at 10 mpk in the bleomycin-induced lung fibrosis model. (a) Schematic of prophylactic dosing paradigm. Bleomycin-treated mice were dosed through intravenous injection with saline, MRG-201 on days 3, 7, 10 and 13. On day 14, animals were sacrificed, and lung tissue analysed. (b) Mean % total lung collagen quantified by Orbit machine learning image analysis software in bleomycin-induced mice treated with MRG-201 or MRG-229. (c) qPCR analysis of downregulated gene expression levels of a panel of fibrosis-associated genes in lung harvested from bleomycin-induced mice treated with either MRG-201 or MRG-229. C. (d) qPCR analysis of downregulated gene expression levels of a panel of fibrosis-associated genes in lung harvested from bleomycin-induced mice treated with either saline or the MRG-229-unconjugated with all the stability modifications but lacking the BiPPB conjugate. Statistical analyses Ordinary one-way ANOVA (***P<0.001) were performed in GraphPad Prism. mpk - mg/Kg.