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. 2022 Oct 21;13:6266. doi: 10.1038/s41467-022-33844-2

Fig. 1. DNMT3A expression is downregulated in IBD patients.

Fig. 1

a Relative mRNA expression of DNMT3A, DNMT3B, and DNMT1 in biopsies from healthy controls (n = 30), CD non inflamed (non infl.) (n = 30), UC noninflamed (non infl.) (n = 30), CD inflamed (inf.) (n = 30), and UC inflamed (inf.) (n = 30). Beta-actin was used as housekeeping gene. b Gene expression analysis of DNMT3A, CCND1, and CXCL10 in human colonic organoids derived from healthy controls (n = 3), CD non infl. (n = 3), and CD inf. (n = 3) patients. Beta-actin was used as housekeeping gene. c Immunoblot and quantification of DNMT3A protein expression in human colonic organoid lysates derived from healthy controls (n = 4), CD non infl. (n = 5), and CD inf. (n = 6) patients. GAPDH protein expression was use for normalization. The values represent mean ± SEM. Statistical analysis was performed using one-way ANOVA together with Tukey post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.