Fig. 8. Apical-junctional proteins are downregulated in Dnmt3a^ΔIEC mice during inflammation.

a Colon mRNA expression levels of Ocln, Ctnnb1, Cdh1, and ZO-1 in crypts isolated from Dnmt3a^ΔIEC and Dnmt3a^fl/fl mice (qRT-PCR) in chronic DSS colitis at day 30 of the experiment (n = 6). Murine beta-actin was used as housekeeping gene. b Representative images and quantification of ZO-1 fluorescence intensity from colonic tissue sections of Dnmt3a^ΔIEC and Dnmt3a^fl/fl mice in chronic DSS colitis at day 30 of the experiment. ZO-1 is depicted in green while nuclei in light blue. Each dot represents each animal (n = 5). c Ocln, Ctnnb1, and Cdh1 mRNA expression levels in crypts isolated from colon tissues derived from Dnmt3a^ΔIEC and Dnmt3a^fl/fl mice during acute inflammation (day 5, n = 5) and recovery phase (day 12, n = 7/8). Murine beta-actin was used as housekeeping gene. d Schematic workflow (left panel) and SEAP production (right panel) of HEK-Blue TLR4 reporter assay. HEK-Blue TLR4 reporter cells were stimulated with serum derived from mice subjected to early inflammation (day 5, n = 5) and recovery phase (day 12, n = 6/8). Data are shown as fold change normalized with serum from untreated animals. Each dot represents a different mouse donor. The values represent mean ± SEM. Graphical elements modified from refs. 17, 74. Statistical analysis was performed using two-tailed t-test with Mann–Whitney correction (a, b) or one-way ANOVA together with Tukey post hoc test (c, d). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.