Table 2.

Validation parameters of vitamin B₁, vitamin B₂, nicotinic acid, nicotinamide, and pyridoxine.

Analytical parameters	Vitamin B1	Vitamin B2	Nicotinic acid	Nicotinamide	PN
Linear range (μg/mL)	0.05–0.2	0.05–0.2	1–10	1–10	0.01–0.5
Correlation coefficient (r2)	0.998	0.998	0.999	0.998	0.9998
LOD (μg/mL)	0.005	0.004	0.006	0.010	0.0011
LOQ (μg/mL)	0.018	0.017	0.020	0.029	0.004
Repeatability limit (%)	2.0	1.2	2.3	2.9	2.8
Reproducibility limit (%)	2.3	1.5	2.6	3.2	4.8
Recovery (%)	96.80–99.30	97.30–101.20	98.60–100.30	97.80–102.50	93.20–96.80

PN, pyridoxine; LOD, limit of detection; LOQ, limit of quantification.