Figure 10.

DA neuron expression of Shh at different developmental stages. scRNA-seq data of DA neurons from different age groups of mice show that adult SN DA neurons (magenta group > P90 in panel (A)) have no or very low levels of Shh expression (B), while they show abundant expression of other DA neuron markers such as Th (C) and Nr4a2 (D)

Note that the Y axis scales for Th and Nr4a2 are larger than the scale for Shh expression. Plot was generated using scRNA-seq data obtained from Tiklová et al., a previous study (Tiklová et al., 2019) with permission from Dr. Thomas Perlmann.