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. 2022 Oct 7;25(11):105278. doi: 10.1016/j.isci.2022.105278

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Shh-expressing cells in the young adult mouse brain labeled by ShhCreER-tdTomato line treated with TAM at P30-P35 age

tdTomato + cells at 25 days post TAM depicting Shh-expressing cells at P30-P35 stage are observed in (A–D) cortical PV + neurons and (E–G) striatal PV + neurons, but not CHAT + neurons. tdTomato + cells are also observed in (H–K) MS/VDB neurons but mostly not in CHAT + cholinergic neurons. tdTomato + neurons are also present in the SN (NeuN+, image not shown) but are not colocalized to TH + DA neurons (M and N). Consistent with this data, tdTomato + neuronal terminals in the PFC (O) and STR (P) do not colocalize with TH + fibers. Scale bar = 50um).