Table 1.

Primer/probe sets used in qRT-PCR

Primer/probe set:
Hmbs:	Forward Primer:	5′-TCCCTGAAGGATGTGCCTAC-3′
	Reverse Primer:	5′-ACAAGGGTTTTCCCGTTTG-3′
	Probe:	Universal Probe Library: Probe 79 - Roche
CHAT:	Forward Primer:	5'- GGTTCGGTGCGTAACAGC-3′
	Reverse Primer:	5'- GCGATTCTTAATCCAGAGTAGCA-3′
	Probe:	Universal Probe Library: Probe 108 - Roche
VCHAT	Forward Primer:	5'- AGAGCCCTACCCTGATCTCTG-3′
	Reverse Primer:	5'- CAAGTAGGCGCTGGCATTAG-3′
	Probe:	Universal Probe Library: Probe 70 - Roche
shh:	Forward Primer:	5′-ACCCCGACATCATATTTAAGGA-3′
	Reverse Primer:	5′-TTAACTTGTCTTTGCACCTCTGA-3′
	Probe:	Universal Probe Library: Probe 32 - Roche
Gli	Forward Primer:	5′-CTGACTGTGCCCGAGAGTG-3′
	Reverse Primer:	5′-CGCTGCTGCAAGAGGACT-3′
	Probe:	Universal Probe Library: Probe 84 - Roche
Drd1a:	Forward Primer:	5′-TCTGGTTTACCTGATCCCTCA-3′
	Reverse Primer:	5′-GCCTCCTCCCTCTTCAGGT-3′
	Probe:	Universal Probe Library: Probe 82 - Roche
Drd2:	Forward Primer:	5′-CTCTTTGGACTCAACAACACAGA-3′
	Reverse Primer:	5′-AAGGGCACGTAGAACGAGAC-3′
	Probe:	Universal Probe Library: Probe 25 - Roche
GDNF:	Forward Primer:	5′-TCCAACTGGGGGTCTACG-3′
	Reverse Primer:	5′-GACATCCCATAACTTCATCTTAGAGTC-3′
	Probe:	Universal Probe Library: Probe 70 - Roche
PGK1 (gDNA):	Forward Primer:	5′-TACCTGCTGGCTGGATGG-3′
	Reverse Primer:	5′-CACAGCCTCGGCATATTTCT-3′
	Probe:	Universal Probe Library: Probe 108 - Roche
Floxed Shh intron 1 (gDNA):	Forward Primer:	5′-GCTGCCTAGACCAAGAATGT-3′
	Reverse Primer:	5′-TGAGGAAACCCATAGCAACTC-3′
	Probe:	FAM/CTTGTCCTT/ZEN/GACCCACTGCCTACC/3IABkFQ-
Unfloxed Shh exon 3 (gDNA):	Forward Primer:	5′-ATTGGCACCTGGCTGTT-3′
	Reverse Primer:	5′-GCTTTCCCATCAGTTCCTTATTTC-3′
	Probe:	FAM/CGAGACCAT/ZEN/GCATCCCTTGGGAAT/3IABkFQ