Table 2.
Primer sequences.
| Gene | Primer Sequence 5′-3′ | GenBank | Product Length/bp |
|---|---|---|---|
| β-Actin | F:5ʹ- CAAAAGCCAACAGAGAGAAGAT -3ʹ | NM_205518.1 | 138 |
| R:5ʹ- CATCACCAGAGTCCATCACAAT-3ʹ | |||
| Occludin | F:5ʹ- AGTTCGACACCGACCTGAAG -3ʹ | NM_205128.1 | 124 |
| R:5ʹ- TCCTGGTATTGAGGGCTGTC -3ʹ | |||
| Claudin-1 | F:5ʹ- TATGGCAACAGAGTGGCTCG -3ʹ | NM_001013611.2 | 171 |
| R:5ʹ- GCTGGGTGGGTAGGATGTTT -3ʹ | |||
| ZO-1 | F:5ʹ- AAGTGTTTCGGGTTGTGGAC -3ʹ | XM_413773.4 | 160 |
| R:5ʹ- GCTGTCTTTGGAAGCGTGTA -3ʹ | |||
| MyD88 | F:5ʹ- GGGATGTCTTGCCAGGAACG -3ʹ | NM_001030962.4 | 186 |
| R:5ʹ- TGCACTTGACCGGAATCAGC -3ʹ | |||
| TLR4 | F:5ʹ- TTCGGTTGGTGGACCTGAATCTTG -3ʹ | NM_001030693.1 | 114 |
| R:5ʹ- ACAGCTTCTCAGCAGGCAATTCC -3ʹ | |||
| NF-κB | F:5ʹ- CCACAACACAATGCGCTCTG -3ʹ | NM_205129.1 | 112 |
| R:5ʹ- AACTCAGCGGCGTCGATG -3ʹ | |||
| IL-1β | F:5ʹ- GGAGCAGGGACTTTGCTGAC -3ʹ | NM_204524.1 | 130 |
| R:5ʹ- AAGGACTGTGAGCGGGTGTA -3ʹ | |||
| IL-4 | F:5ʹ- TCTTCCTCAACATGCGTCAG -3ʹ | NM_001007079.1 | 127 |
| R:5ʹ- TGGTGGAAGAAGGTACGTAGG -3ʹ | |||
| IL-6 | F:5ʹ- AAATCCCTCCTCGCCAATCT -3ʹ | NM_204628.1 | 106 |
| R:5ʹ- CCCTCACGGTCTTCTCCATAAA -3ʹ | |||
| IL-10 | F:5ʹ- CAGACCAGCACCAGTCATCA -3ʹ | NM_001004414.2 | 163 |
| R:5ʹ- TCCCGTTCTCATCCATCTTCTC -3ʹ | |||
| IFN-γ | F:5ʹ- CTTCCTGATGGCGTGAAGA -3ʹ | NM_205149.1 | 127 |
| R:5ʹ- GAGGATCCACCAGCTTCTGT -3ʹ |