Fig. 5.
Nuclear envelope invaginations in hepatocytes from L-CKO mice are type 1 nucleoplasmic reticula. A: Representative confocal fluorescence photomicrograph of a hepatocyte from a L-CKO mouse labeled with anti-lamin A/C Abs (red), Ab MAb414 that recognizes several nuclear pore complex proteins (green) and DAPI (blue); overlap of red and green appears yellow. Pore complexes are at the periphery and excluded from the interior of the nucleus. Scale bar: 10 μm. B: 3D reconstruction of the nucleus shown in A labeled with anti-lamin A/C Abs (red), Ab MAb414 (green), and DAPI (blue). Top panel shows the lamin A/C signal (red), middle panel the pore complex signal (green), and bottom panel those signals plus DAPI (blue) together, with overlap of red and green appearing yellow. Lamin A/C is located in the nuclear interior and the periphery while pore complexes are only at the periphery and excluded from the interior of the nucleus. Scale bar: 10 μm. C: Representative confocal fluorescence photomicrograph of a hepatocyte from a L-CKO mouse labeled with Abs against TRAPα, a marker of the ER/outer nuclear membrane (red), BODIPY (green), and DAPI (blue). TRAPα is essentially excluded from the nucleus. Scale bar: 10 μm. D: Representative 3D reconstruction of a hepatocyte from a L-CKO mouse labeled with Abs against TRAPα, (red), BODIPY (green), and DAPI (blue). Scale bar: 10 μm. TRAPα, translocon-associated protein α; L-CKO mice, Alb-Cre;Lap1fl/fl mice.