Fig. 7.
Analysis of nuclear LDs in hepatocytes isolated from high-fat diet–fed and fasted L-CKO mice. A: Percent change of initial body mass of mice while on a high-fat diet. Control (Lap1fl/fl) and L-CKO mice at 8 weeks of age were fed a high-fat diet for 8 weeks, and body mass was measured weekly; n = 5–7 mice per group. Each circle and square indicate mean values of body mass. Error bars indicate SEM. B: Liver to body mass ratios of control and L-CKO mice after 8 weeks on a high-fat diet; n = 4–7 mice per group. Each circle and square indicate value from an individual mouse, rectangular bars show means, and error bars indicate SEM; ∗∗P < 0.01 by two-tailed Student’s t test. C: Representative widefield fluorescence photomicrographs of hepatocytes from L-CKO mice stained with BODIPY (green) and DAPI (blue). Hepatocytes were isolated from chow-fed (left panel) or high-fat diet–fed (right panel) L-CKO mice. Arrows indicate nuclei containing LDs. Scale bar: 25 μm. D: stacked column graph with different colors representing the percentages of hepatocyte nuclei containing the indicated numbers of nuclear LDs. We analyzed a total of 112 (chow diet) and 179 (high-fat diet) nuclei of hepatocytes cultured on three different coverslips (n=3 per group). The numbers at the top of the graphs indicate the mean percentages of hepatocyte nuclei with two or more nuclear LDs. These values and those within graphs are means ± SEM. ∗∗∗P < 0.001 for percentage of hepatocyte nuclei with two or more nuclear LDs by two-tailed Student’s t test. E: Representative widefield fluorescence photomicrographs of hepatocytes from L-CKO mice stained with BODIPY (green) and DAPI (blue). Hepatocytes were isolated from mice fed a normal chow diet (left panel) or mice fasted for 24 h (right panel). Arrows indicate nuclei containing LDs. Scale bar: 25 μm. F: Stacked column graph with different colors representing the percentage of nuclei containing the indicated numbers of nuclear LDs in hepatocytes isolated from L-CKO mice fed normally or after 24 h of fasting. We analyzed a total of 479 (fed) and 599 (fasted) nuclei of hepatocytes cultured on three different coverslips (n=3 per group). The numbers on the top of the graphs indicate the mean percentages of hepatocyte nuclei with two or more nuclear LDs. Data are shown as means ± SEM. ∗∗∗P < 0.001 for percentage of hepatocyte nuclei with two or more nuclear LDs by two-tailed Student’s t test. L-CKO mice, Alb-Cre;Lap1fl/fl mice; LDs, lipid droplets