Fig. 9.

Immunoblotting of fibrinogen in the ipsilateral hemisphere and quantification of intracerebral hemorrhage. $\beta$-actin was used for immunoblotting housekeeping genes (normal saline treatment for sham; Rose Bengal (RB) with thrombin treatment for T+RB; rtPA treatment for rtPA; rmDPPs with magneto-sonothrombolytic approach for +M+US group). (a-b) Typical immunoblotting and quantification of fibrinogen in the RB and T+RB groups, showing stable platelet deposits were observed in the ipsilateral hemisphere compared with RB alone (p = 0.047). (c-d) Typical immunoblotting and quantification of fibrinogen expression in the sham, T+RB, rtPA, and +M+US groups, showing that rtPA treatment showed not only an unstable fibrinolytic potential (standard deviation: 0.507, $n=5$), but also no significant difference was detected when compared to the T+RB (T+RB for $n=12$; rtPA for $n=3$; $p=0.16$). There was no significant difference between the rtPA and +M+US groups ($p=0.80$). (e) Typical image of intracerebral HT after delayed treatment with either drug or particles with a magneto-sonothrombolytic approach. (f-g) Regressed cyanmethemoglobin standard curve and quantification of intracerebral HT, showing delayed treatment of rtPA induced the HT ($n=4$), whereas the +M+US group did not present any HT ($n=3$)