Table 2.
Antibodies used for western blot assays
| Antibody | Expected molecular weight (kDa) | Concentration | Manufacturera |
|---|---|---|---|
| α-GCN1 primary—rabbit | 293 | 1:1000 | Abcam (ab86139) |
| α-eIF2α primary—mouse | 36 | 1:250 | Abcam (ab5369) |
| α-phospho-eIF2α primary—rabbit | 36 | 1:250 | Abcam (ab32157) |
| α-actin primary—mouse | 43 | 1:500 | MilliporeSigma (MAB1501) |
| α-mouse HRP secondary | N/A | 1:1000 or 1:2000 (actin) | MilliporeSigma (12–349) |
| α-rabbit HRP secondary | N/A | 1:1000 | MilliporeSigma (12–348) |
eIF2α Eukaryotic translation initiation factor 2 subunit alpha, HRP horseradish peroxidase
aAbcam, Cambridge, UK; MilliporeSigma, Burlington, MA, USA