Skip to main content
. 2022 Oct 21;15:383. doi: 10.1186/s13071-022-05461-x

Table 3.

Quantitative reverse transcription PCR analysis primers

Primer Sequence Primer melting temperature (Tm; °C)
gcn1 forward TCGGAGGAGCAAACCAATACG 60
gcn1 reverse TTCTTCAATTTCACGACGCAGG
vg forward ATGCACCGTCTGCCATC 60
vg reverse GTTCGTAGTTGGAAAGCTCG
rps7 forwarda TCAGTGTACAAGAAGCTGACCGGA 60
rps7 reversea TTCCGCGCGCGCTCACTTATTAGATT
β-actin forwarda GACTACCTGATGAAGATCCTGAC 60
β-actin reversea GCACAGCTTCTCCTTAATGTCAC

β-actin Beta-actin, vg vitellogenin, rps7 ribosomal protein S7

aInternal reference genes