Figure 3.

LINC02418 is upregulated in CRC chemoresistant cell lines and responsible for the chemoresistance of CRC. (A) LINC02418 was upregulated in HCT8/5-Fu and HCT8/DDP cells detected and validated by RNA-sequencing and qRT-PCR, respectively. (B) The expression of LINC02418 was knocked down by three different siRNAs in two chemoresistant cell lines. Cell viability and IC₅₀ values of HCT8/5-Fu (C) and HCT8/DDP cells (D) with LINC02418 knockdown were assessed by MTT assay. (E) Knockdown LINC02418 in chemoresistant cells increased cell apoptosis rates. (E) LINC02418 knockdown led to a decrease in the S phase in HCT8/5-Fu (F) and HCT8/DDP cells (G). Data are shown as mean ± SD, and the results represent three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.