Figure 4.

MiR-372-3p is downregulated in CRC chemoresistant cell lines and responsible for the chemoresistance of CRC. (A) MiR-372-3p was lowly expressed in HCT8/5-Fu and HCT8/DDP cells detected and validated by RNA-sequencing and qRT-PCR, respectively. (B) The expression of miR-372-3p was detected when transfected with miR-372-3p mimics or NC mimics in two chemoresistant cell lines. Cell viability and IC₅₀ values of HCT8/5-Fu (C) and HCT8/DDP cells (D) transfected with miR-372-3p and NC mimics were detected. (E) The proliferation capacities were inhibited in miR-372-3p overexpressing chemoresistant cells. (F) Flow cytometry assays suggested that the percentage of apoptotic chemoresistant cells was increased by miR-372-3p overexpression. (G) Overexpressed miR-372-3p led to the changes in cell cycle distribution in chemoresistant cells. Data are shown as mean ± SD, and the results represent three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.