Figure 5.

EPHA2 is upregulated in CRC chemoresistant cell lines and related to the chemoresistance of CRC. (A) EPHA2 was highly expressed in HCT8/5-Fu and HCT8/DDP cells detected and validated by RNA-sequencing and qRT-PCR and western blotting, respectively. (B) The activity of EPHA2 was inhibited when treated with ALW-II-41-27 for 72 h. Cell viability and IC₅₀ values of HCT8/5-Fu (C) and HCT8/DDP cells (D) treated with 1 µM of ALW-II-41-27 and dimethyl sulfoxide (DMSO) for 72 h were assessed by MTT assay. (E) The proliferation capacities, (F) Apoptotic rates and (G) distributions of the cell cycle were assessed in HCT8/5-Fu and HCT8/DDP cells treated with either DMSO or 1 µM of ALW-II-41-27 for 72 h. Data are shown as mean ± SD, and the results represent three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.