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. 2022 Oct 12;7(5):637–647. doi: 10.1089/can.2021.0100

FIG. 1.

FIG. 1.

5-epi induces de novo synthesis of long chain Cer species in CRC cells. (A) Diagrammatic representation of the de novo sphingolipid synthesis pathway and metabolic fates of Cer. Enzymes of the de novo synthesis pathway are colored red. Enzymes that attenuate Cer accumulation are colored blue. (B) LC/MS/MS analysis of the sphingoid bases, dhSph and Sph, in DLD-1 cells treated with the indicated concentrations of 5-epi, alone or in combination with the SphK inhibitor PF-543 (5 μM), for 48 h (*p<0.001). (C) LC/MS/MS analysis of the respective 1-phosphates (*p<0.0001, #p<0.005, p<0.002). (D) LC/MS/MS analysis of major Cer species in DLD-1 cells treated with 5-epi±PF-543 as indicated, for 48 h (*p<0.0001, #p=0.0057). 5-epi, (±) 5-epi CP 55,940; Cer, ceramide; CRC, colorectal cancer; dhSph, dihydrosphingosine; LC/MS/MS, Liquid Chromatography - Tandem Mass Spectrometry; Sph, sphingosine; SphK, sphingosine kinase.