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. 2022 Oct 7;24(5):304–313. doi: 10.1089/cell.2021.0200

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© Lena Böhnke, et al., 2022. Published by Mary Ann Liebert, Inc.

This open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 4. — (A) Cell type of IM1. Weak βIII-tubulin staining, no neuronal morphology. (B) Cell type of IM2. Strong βIII-tubulin staining, no neuronal morphology. (C) iN. Strong βIII-tubulin staining, neuronal morphology. (D–F) Quantification of immunofluorescent images of IM1 (D), IM2 (E), and iNs (F) in CL01 and CL02 for βIII-tubulin/DAPI during conversion. Significance: t-test; *: p ≤ 0.05; ***: p ≤ 0.01; ****: p ≤ 0.001. Staining: βIII-tubulin (red), DAPI (blue). IM1, intermediate stage 1; IM2, intermediate stage 2.