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. 2022 Sep 20;149(18):dev200860. doi: 10.1242/dev.200860

Fig. 1.

Fig. 1.

Lats1/2 double conditional knockout (DCKO) mutant embryos have craniofacial phenotypes with severe NTDs. (A) Bright-field images of E9.5 and E10.5 embryos. Compared with controls (a-d,i-l), DCKO embryos have smaller heads and parts of the forebrain, midbrain and hindbrain missing (e-h,m-p). DCKO embryos have open cranial neural tubes (NTs) (f,h,n,p, outlined). Boxed areas are shown at higher magnification (right). Scale bars: 500 µm. (B) 3D reconstruction of microcomputed tomographic (µCT) imaging of E10.5 embryos. Compared with control embryos (a-c), DCKO embryos have smaller heads and abnormal NTs (e-g,i-k, outlined; arrows indicate open NT). Coronal µCT slices (higher magnification on right) illustrate the NT ventricular space in control embryos (asterisk, d), which is absent in the DCKO embryos (h,l). CNT, closed neural tube; fb, forebrain; hb, hindbrain; mb, midbrain; ONT, open neural tube.