Fig. 6.

Yap/Taz haploinsufficiency reduces cell infiltration and partially recovers neuroepithelial cell architecture in Lats1/2-deficient neural tubes. (A) Bright-field images of Lats1/2 DCKO embryos with Yap/Taz heterozygosity (haplo embryos), which are phenotypically like Lats1/2 DCKO embryos. Scale bars: 500 μm. (B) H&E staining of coronal sections at E10.5. Neuroepithelial cell organization is partially recovered in haplo NTs, while cell infiltration into the ventricle is still detected (black asterisk). Boxed areas are shown at higher magnification (right). (C) Immunostaining of proliferation marker pHH3 show mitotic cells on the apical edge of the polarized neuroepithelium for control and haplo NTs (dotted line), in contrast to DCKO with mitotic cells along the apicobasal axis. Nuclei stained with DAPI (blue). Scale bars: 100 μm. Outline delineates neuroepithelium, with yellow lines indicating apical edge and blue lines indicating basal edge. (D) Quantification of pHH3⁺ cells in the NT (control, n=6; DCKO, n=7; haplo, n=4). Data compared using one-way ANOVA followed by Tukey's multiple comparisons test. NT, neural tube; ns, not significant. All error bars represent s.e.m.