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. 2022 Jul 27;29(6):1076–1087. doi: 10.1007/s12282-022-01389-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Trop-2 expression in cell lines. a Exponentially growing normal breast epithelial MCF-10A cells and the indicated breast cancer cells were subjected to immunoblotting with the indicated antibodies. Tubulin was included as a loading control. b MCF-7 and T47D cells were transfected with control siRNA (siCTR) or two different siRNA specific to Trop-2 (siTrop2), and subjected to immunoblotting with anti-Trop-2 antibody. c The indicated breast cancer cells in their exponentially growing state were subjected to RT-qPCR. Relative Trop-2 mRNA levels from triplicate experiments are shown with standard deviation (S.D)