Fig. 5.

Trop-2 is transactivated by TFEB in a manner dependent on a tandem E-box motif. a Sequence of human TACSTD2 (Trop-2) 5′-UTR. E-box consensus sequences are shown in bold. b Schematic diagram of the luciferase reporter constructs fused to TACSTD promoter used in the study. The mutated residues (underline) in the tandem E-box motif in Luc2-EBmt are shown below. c HEK293T cells were transfected with control luciferase reporter vector (V) or indicated reporter constructs (Luc1, 2, and 3) with either empty pcDNA3.1 vector (-) or pcDNA3.1-TFEB. After 48 h the cell lysates were subjected to a luciferase assay. d Luciferase assay was carried out as in c with a reporter mutated in the tandem E-box motif (EBmt) as indicated. e Luciferase assay was carried out as in c with additional GSK3β expression as indicated. Relative luciferase activities from three independent experiments are shown as averages ± S.D. Statistical significances were calculated using one-way ANOVA