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. 2022 Oct 22;13:6295. doi: 10.1038/s41467-022-34128-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Images of silk fibers observed under a fluorescence microscope and b laser confocal microscope. EGFP fluorescence localization of SER3 recombinant protein synthesized by the PSG in silk fiber. c Transmission electron micrograph of a longitudinal silk fiber section. SER3 protein synthesized by the PSG is dispersed in the fibrils of the silk fiber. d Immunofluorescence localization of P25 protein in silk fiber. in Fig. 2a to Fig. 2d: WT-L and SER-L, longitudinal section of silk fiber; WT-C and SER-C, cross-section of silk fiber; R-silk, raw silk; D-silk, degummed silk. SF, fibroin layer of silk. SS, sericin layer of silk. SM, sericin protein microsomes in the silk fibroin fibrils. Vac, vacuoles. e Sericin content of cocoon silk was determined by a classical degumming method. Data were presented as mean ± SEM, and the unpaired t-test analysis was used for the comparison between the two groups. n = 3 cocoons. f The content of SER3 protein in cocoon silk is determined by western blotting; P25 is an internal reference. n = 3. EGFP localization showed that the recombinant SER3 protein had haploid and dimer 2 types, and dimer was the main type. Data were presented as mean ± SEM. Image data are representative of three independent experiments unless otherwise stated.