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. 2022 Oct 22;13(10):894. doi: 10.1038/s41419-022-05327-1

Fig. 6. MMP1 is important for maintenance of latent infection in epithelial cells.

Fig. 6

MMP1 restricts EBV lytic reactivation and provides growth-supportive properties in infected epithelial cell lines. A MMP1 knocked down NPC43 and C17 cells were constructed by lentiviruses and selected by puromycin for three weeks. GFP fluorescence was determined in the selected cells. B The expression of endogenous MMP1 was determined by qRT-PCR. C Quantification of EBV lytic gene BZLF1 and BRLF1 mRNA from three experiments is shown. D Relative EBV-GFP DNA copy numbers were quantified as a measure of EBV load released from NPC43-shMMP1 and C17-shMMP1 before and after the reactivation with TPA and Butyrate. EBV DNA copy number was detected by real-time qPCR. E Cell proliferation fluorescence microscopy images of NPC43-shControl, NPC43-shMMP1, C17-shControl and C17-shMMP1 after 5 days. F Cell growth curves of NPC43 and C17 cells determined by cell count after MMP1 knockdown at different days.