Table 2.
Functional properties of chimeric anti-PD-1 monoclonal antibodies.
| Chimeric antibodies | PD-1 binding activity, EC50 (ng/ml) | PD-1/PD-L1 binding blockade activity, IC50 (ng/ml) | Binding kinetics | T cell activation | |||
|---|---|---|---|---|---|---|---|
| kon (1/Ms) | koff (1/s) | KD (nM) | IFN-γ, EC50 (ng/ml) | IL-2, EC50 (ng/ml) | |||
| xCUSB0103 | 18.90 | 593.4 | 6.53E + 05 | 2.92E−04 | 0.448 | 13.6 | 55.7 |
| xCUSB0123 | 26.59 | 956.7 | 2.98E + 05 | 3.66E−04 | 1.230 | N/A | N/A |
| xCUSB0124 | 56.98 | 2,929 | 1.54E + 05 | 9.12E−04 | 5.920 | N/A | N/A |
| xCUSB0126 | 23.71 | 429.6 | 4.93E + 05 | 2.85E−04 | 0.577 | 77.7 | 219.1 |
| xCUSB0129 | 18.67 | 454.8 | 4.71E + 05 | 2.93E−04 | 0.621 | 78.0 | 244.6 |
| KEYTRUDA | 10.33 | 227.7 | 6.71E + 05 | 4.019E−3 | 5.983 | 5.0 | 10.9 |
| OPDIVO | 10.57 | 460.9 | 1.92E + 05 | 1.397E−3 | 7.274 | 9.1 | 44.2 |
N/A Not available.