. 2022 Oct 22;12:17792. doi: 10.1038/s41598-022-20560-6

Table 2.

Functional properties of chimeric anti-PD-1 monoclonal antibodies.

Chimeric antibodies	PD-1 binding activity, EC₅₀ (ng/ml)	PD-1/PD-L1 binding blockade activity, IC₅₀ (ng/ml)	Binding kinetics			T cell activation
Chimeric antibodies	PD-1 binding activity, EC₅₀ (ng/ml)	PD-1/PD-L1 binding blockade activity, IC₅₀ (ng/ml)	k_on (1/Ms)	k_off (1/s)	K_D (nM)	IFN-γ, EC₅₀ (ng/ml)	IL-2, EC₅₀ (ng/ml)
xCUSB0103	18.90	593.4	6.53E + 05	2.92E−04	0.448	13.6	55.7
xCUSB0123	26.59	956.7	2.98E + 05	3.66E−04	1.230	N/A	N/A
xCUSB0124	56.98	2,929	1.54E + 05	9.12E−04	5.920	N/A	N/A
xCUSB0126	23.71	429.6	4.93E + 05	2.85E−04	0.577	77.7	219.1
xCUSB0129	18.67	454.8	4.71E + 05	2.93E−04	0.621	78.0	244.6
KEYTRUDA	10.33	227.7	6.71E + 05	4.019E−3	5.983	5.0	10.9
OPDIVO	10.57	460.9	1.92E + 05	1.397E−3	7.274	9.1	44.2

N/A Not available.