Fig. 3. Atp8b1 reverses the impairment of efferocytosis and dysregulation of phospholipid metabolism in acinar cells during CP development.

AdAtp8b1 was used to induce Atp8b1 overexpression in the pancreas of PRSS1^Tg mice. The pancreases of PRSS1^Tg mice were infected with adenoviral vectors harbouring a scrambled adRNA for the negative control (NC). A Structural alterations and fibrosis in pancreatic tissues from adAtp8b1 and NC groups PRSS1^Tg mice treated (+) or untreated (−) with caerulein were evaluated by H&E staining and Masson’s trichrome staining, respectively. B The levels of IL-1β, IL-6, and TNF-α in pancreatic tissues from caerulein-treated and untreated PRSS1^Tg mice were quantitatively analyzed by ELISA. C α-SMA expression in pancreatic tissues from caerulein-treated and untreated PRSS1^Tg mice was detected by immunofluorescence staining, the mean gray values calculated by ImageJ were used to quantitatively analyze the degree of fibrosis. The mean gray values was calculated using integrated density divided by area. D Apoptosis and F4/80 expression were detected by a TUNEL assay and immunofluorescence staining, respectively, in pancreatic tissues from caerulein-treated and untreated PRSS1^Tg mice. The mean gray values calculated by ImageJ were used to quantify these parameters. E The mRNA expression of CD206 and ARG1 in the pancreatic tissues from adAtp8b1-treated and untreated PRSS1^Tg CP mice was measured by RT-PCR. F Immunohistochemical staining was used to detect CD206 and ARG1 expression in pancreatic tissues from adAtp8b1-treated and untreated PRSS1^Tg CP mice. G The concentration of LPC in pancreatic tissues from adAtp8b1-treated and untreated PRSS1^Tg CP mice was assessed by LC–MS/MS. PRSS1^Tg (+) and PRSS1^Tg (−) represented mice treated and untreated with caerulein, respectively. The data are presented as the means ± SDs. ns no significant difference; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Scale bars = 100 µm.