FIG. 1.

NK-1R mRNA expression was analyzed by RT-PCR of equalized input RNA from IM-9 cells, LPMC, resting PBMC, PBMC activated with PHA, monocytes, MDM, and negative controls (lanes 1 to 7, respectively). HaeIII-digested φX174 DNA size markers were used (lane M). Gel A shows mRNA-specific amplification products (202 bp) for β-actin to control for amplification efficiency. Gel B shows mRNA-specific amplification products for the short isoform of NK-1R (438 bp). Gels C and E show mRNA-specific amplification products (295 bp) from primary-round RT-PCR for NK-1R using 1% (C) and 10% (E) cDNA. Gels D and F show mRNA-specific amplification products for NK-1R (242 bp) from secondary-round nested RT-PCR using 1% (D) and 10% (F) cDNA.