FIG. 3.
Quantitation of NK-1R mRNA expression in a representative LPMC isolation. Competitive standard transcripts were spiked into the aliquoted LPMC RNA sample at concentrations ranging from 4.0 × 105 to 4.1 × 103 (2.5-fold dilution series), followed by NK-1R mRNA-specific RT-PCR (lanes 1 to 6). HaeIII-digested φX174 DNA size markers were used (lane M). Equivalence (∗) is seen at 6.4 × 104 NK-1R mRNA molecules in which target (295 bp) and competitive-standard (228 bp) RT-PCR products are of equal band intensity. When adjusted for the amount of total RNA in the assay, this represents a level of expression of 7.5 × 105 NK-1R mRNA transcripts/μg of total RNA isolated.