Table 2.
Cumulative amount and flux of CL, and residual CL on the skin surface 24 h after CL gel application
| Skin sample | Cumulative amount permeated in RS (ng/cm2) | Applied dose permeated into RS (%) | Peak CL flux into RS (ng/cm2/h) | Residual CL on skin surface (ng) | Residual CL on skin surface (% of applied dose) |
|---|---|---|---|---|---|
| Epidermal membrane (mean ± SE) | |||||
| 0.016% CL gel | 73.3 ± 14.1b | 4.6 ± 0.9b | 10.8 ± 3.6b | 21.0 ± 4.7c | 1.3 ± 0.3c |
| Blanka | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | ||
| Dermatomed skin (mean ± SD) | |||||
| 0.016% CL gel | 40.4 ± 9.4d | 2.5 ± 0.5d | 5.2 ± 1.1d | BLQ | BLQ |
| Blanka | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | ||
BLQ below the limit of quantification, CL chlormethine, RS receptor solution, SD standard deviation, SE standard error
aExperiment run with no CL formulation applied
bThree donors, 19 replicates; receptor solution was not collected for 2 replicates, which were therefore excluded from the analysis
c Three donors, 12 replicates; only 12 of the possible 21 replicates had quantifiable residual CL on the membrane surface
dOne donor, 6 replicates; 1 replicate with an abnormal permeation profile was removed