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. 2022 Oct 22;27:94. doi: 10.1186/s11658-022-00364-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — circ_0025033 knockdown hampered the malignant behavior of ovarian cancer cells in glutamine metabolism. A–M SKOV3 and A2780 cells were transfected with si-NC or si-circ_0025033. A qRT-PCR analysis of circ_0025033. B and C CCK-8 and EdU analysis of cell viability and DNA synthesis. D Apoptosis rate analyzed by flow cytometry analysis. E and F Cell invasion measured by transwell assay. G Tube formation assay for evaluating angiogenesis activity. H–J Western blot analysis of c-Myc and MMP9 levels. K–M Glutamine consumption, α-ketoglutarate production, and glutamate production measured via glutamine, α-ketoglutarate, and glutamate assay kits, respectively. **P < 0.01, ***P < 0.001, ****P < 0.0001