| Antibodies |
| Anti-VIPR1 |
ThermoFisher |
Cat#: PA3-113; RRID: AB_2273050 |
| Anti-β-actin |
Sigma Aldrich |
Cat#: A5316; RRID: AB_476743 |
| Anti-rabbit (western) |
Santa Cruz Biotechnology |
Cat#:SC-2054; RRID: AB_631748 |
| Anti-mouse |
Santa Cruz Biotechnology |
Cat#: SC-2005; RRID: AB_631736 |
| Anti-parvalbumin |
Swant |
Cat#: PV235; |
| Anti-GFP |
Abcam |
Cat#: ab13970; RRID: AB_300798 |
| Anti-GABA |
Sigma Aldrich |
Cat#: A2052; RRID: AB_477652 |
| Alexa fluor 568 goat anti-mouse |
ThermoFisher |
Cat#: A11004; RRID: AB_2534072 |
| Alexa fluor 488 goat anti-chicken |
ThermoFisher |
Cat#: A11039; RRID: AB 2534096 |
| Alexa fluor 488 goat anti-rabbit |
ThermoFisher |
Cat#: A11008; RRID: AB_143165 |
| Bacterial and virus strains |
|
pAAV-hDLX-Vipr1-T2A-eGFP
|
This paper |
n/a |
|
pAAV-hDLX-Vipr1-T2A-tdTomato
|
This paper |
n/a |
|
rAAV-hSyn-DIO-hM4Di-IRES-mCitrine
|
Addgene |
Plasmid#: 50455; RRID: Addgene_50455 |
|
AAV-CAG-Flex-Vipr1-GFP
|
This paper |
n/a |
|
AAV-CAG-Flex-GFP
|
This paper |
n/a |
| Biological samples |
| Human superior temporal lobe samples |
NIH NeuroBioBank |
n/a |
| Chemicals, peptides, and recombinant proteins |
| PG-97-269 |
Bachem |
Cat#: 4048647 |
| Critical commercial assays |
| mirVana RNA isolation kit |
ThermoFisher |
Cat#: AM1561 |
| TruSeq Stranded Total RNA Library Prep |
Illumina |
Cat#: 20020599 |
| Aurum Total RNA Mini kit |
Bio-Rad |
Cat#: 7326820 |
| Deposited data |
| RNA-seq (mouse cortical interneurons) |
NCBI GEO |
Accession #: GSE195491
|
| RNA-seq (hIPSC organoids) |
NCBI GEO |
Accession #: GSE195505
|
| Experimental models: Cell lines |
|
NSUN5–GTF2IRD2+/− hIPSC |
This paper |
n/a |
| Experimental models: Organisms/strains |
|
Gtf2ird1 mice |
Young, et al., 2008
|
n/a |
|
CD+/− mice |
Segura-Puimedon et al., 2014
|
n/a |
|
PD+/− mice |
Jackson Laboratory |
Strain#: 023885; RRID:IMSR_JAX:02 3885 |
|
DD+/− mice |
Jackson Laboratory |
Strain#: 023888; RRID:IMSR_JAX:02 3888 |
|
Gtf2i mice |
MMRC |
Stock#: 034666-UCD; RRID: MMRRC_034666-UCD |
|
Vipr1 cKO mice |
This paper |
n/a |
|
Vipr1-OE mice |
This paper |
n/a |
|
Gad2Cre
|
Jackson Laboratory |
Strain#: 019022; RRID: IMSR_JAX: 019022 |
|
PVCre
|
Jackson Laboratory |
Strain#: 017320; RRID: IMSR_JAX: 017320 |
|
Ai14
|
Jackson Laboratory |
Strain#: 007914; RRID: IMSR_JAX: 007914 |
|
Ai93
|
Jackson Laboratory |
Strain#: 024107; RRID: IMSR_JAX: 024107 |
|
CaMKIIαttA
|
Jackson Laboratory |
Strain#: 24108; RRID: IMSR_JAX: 24108 |
|
Scnn1aCre
|
Jackson Laboratory |
Strain#: 009613; RRID: IMSR_JAX:009613 |
| Oligonucleotides |
| See tables in methods. |
|
|
| Recombinant DNA |
| n/a |
|
|
| Software and algorithms |
| pClamp |
Molecular Devices |
n/a |
| miniAnalysis |
Synaptosoft |
n/a |
| Prism |
Graphpad |
n/a |
| Sigmaplot |
Systat |
n/a |
| Fiji |
Schindelin et al., 2012
|
n/a |
| Matlab |
Mathworks |
n/a |
| Ilastik |
Berg et al., 2019
|
n/a |
| OASIS |
Friedrich et al., 2017
|
n/a |
| Linear Decoder |
This paper |
n/a |
